pcb 3023l cell bio lab 1 protein quantification 1
Upon completion of this lab
exercise
, you should be able to
:

Describe how Coomassie brillian
t
blue G
–
250 dye can be used to detect proteins in
solution

Determine how a set of known protein sample concentrations can be utilized to
determine the concentration of an unknown sample through the use of
a Coomassie
colori
metric assay

Identify the unknown sample dilution(s) that allow
s
you to most confidently determine
the protein concentration
of your unknown

Calculate the unknown sample’s concentration taking into consideration dilution factors
,
where needed

Identify pot
ential sources of variation
in the experimental procedure and establish
techniques to help minimize experimental error

Suggest when to use serial vs parallel dilutions and why each can be beneficial
depending
on the
particular experimental conditions
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